Difference between revisions of "Part:BBa K299504"
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Latest revision as of 23:30, 25 October 2010
Expression vector J23100+B0034
This part is licensed under
Creative BioCommons
This is expression vector containing B0034 under J23100 promoter. It can be used for easily expression of any protein-encoding BioBrick in RFC10 format.
Authors
Clonning:
- Ania Puławska
- Milena Bażlekowa
The lab work was supervised by:
- Michał Lower
- Ania Olchowik
Construct design
The device consists of J23100 promoter followed by B0034 RBS. Device was created by annealing of synthetic complementary oligonucleotides followed by subsequent cloning into pSB1A2 vector.
Expression strength
Expression strength of this device is based on measurements of [http://2010.igem.org/Team:Warsaw/Stage1/PromMeas promoter strength] and [http://2010.igem.org/Team:Warsaw/Stage1/RBSMeas RBS performance] done by Team Warsaw 2010.
It's estimated to be 31,34% relative to BBa_K299509, the strongest ExpressIt! vector.
Summary for the whole ExpressIt! family is shown below:
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]