Difference between revisions of "Part:BBa K364328:Experience"

Revision as of 23:21, 25 October 2010

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Please enter how you used this part and how it worked out.

Physiological effects of DAF-12

daf-12 in Caenorhabditis elegans plays a key role in the regulation of development and determination of adult longevity. It has effects also on resistance to oxidative and thermal stress which makes daf-12 the first gene with alleles that can extend or shorten lifespan.

Possible applications of GAL4-DAF-12

By the addition of lipid ligands(unknown), the created system activates the expression of any kind of genes fused to UAS(upstream activation seqence)previously. Also can be used as a lipid sensor by activating reporter genes (e.g. Luciferase).

Applications of BBa_K364328

Picture of gel electrophoresis: Gal4-DAF12 in pCDNA3.1 resulting an insert of 971 bp.

User Reviews

UNIQaf965dbc4a111107-partinfo-00000000-QINU UNIQaf965dbc4a111107-partinfo-00000001-QINU

@@ Line 2: / Line 2: @@
 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 how you used this part and how it worked out.
+===Physiological effects of DAF-12===
+daf-12 in Caenorhabditis elegans plays a key role in the regulation of development and determination of adult longevity. It has effects also on resistance to oxidative and thermal stress which makes daf-12 the first gene with alleles that can extend or shorten lifespan.
+===Possible applications of GAL4-DAF-12===
+By the addition of lipid ligands(unknown), the created system activates the expression of any kind of genes fused to UAS(upstream activation seqence)previously. Also can be used as a lipid sensor by activating reporter genes (e.g. Luciferase).
 ===Applications of BBa_K364328===