Difference between revisions of "Part:BBa K364325:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
how you used this part and how it worked out.
 
how you used this part and how it worked out.
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===Physiological function of PXR===
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Like other type II nuclear receptors, when activated, it forms a heterodimer with the retinoid X receptor, and binds to hormone response elements on DNA which elicits expression of gene products.
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One of the primary targets of PXR activation is the induction of CYP3A4, an important phase I oxidative enzyme that is responsible for the metabolism of many drugs. In addition, PXR up regulates the expression of phase II conjugating enzymes such as glutathione S-transferase and phase III transport uptake and efflux proteins such as OATP2 (SLCO2A1)and MDR1(ABCB1).
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===Possible applications of GAL4-PXR===
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By the addition of lipid ligand, the created system activates the expression of any kind of genes fused to UAS(upstream activation seqence)previously. Also can be used as a lipid sensor by activating reporter genes (e.g. Luciferase).
  
 
===Applications of BBa_K364325===
 
===Applications of BBa_K364325===
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[[Image:Team Debrecen WB pCDNA-Gal4-PXR.jpg|800px|thumb|center|Picture of Western blot: Gal4-PXR composite construct is highly expressed after transferring it into pCDNA3.1]]
 
[[Image:Team Debrecen WB pCDNA-Gal4-PXR.jpg|800px|thumb|center|Picture of Western blot: Gal4-PXR composite construct is highly expressed after transferring it into pCDNA3.1]]
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===User Reviews===
 
===User Reviews===
 
<!-- DON'T DELETE --><partinfo>BBa_K364325 StartReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K364325 StartReviews</partinfo>

Revision as of 23:05, 25 October 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Physiological function of PXR

Like other type II nuclear receptors, when activated, it forms a heterodimer with the retinoid X receptor, and binds to hormone response elements on DNA which elicits expression of gene products.

One of the primary targets of PXR activation is the induction of CYP3A4, an important phase I oxidative enzyme that is responsible for the metabolism of many drugs. In addition, PXR up regulates the expression of phase II conjugating enzymes such as glutathione S-transferase and phase III transport uptake and efflux proteins such as OATP2 (SLCO2A1)and MDR1(ABCB1).

Possible applications of GAL4-PXR

By the addition of lipid ligand, the created system activates the expression of any kind of genes fused to UAS(upstream activation seqence)previously. Also can be used as a lipid sensor by activating reporter genes (e.g. Luciferase).

Applications of BBa_K364325

Picture of gel electrophoresis: Gal4-PXR in pCDNA3.1 resulting an insert of 1181 bp.
Picture of Western blot: Gal4-PXR composite construct is highly expressed after transferring it into pCDNA3.1

User Reviews

UNIQe9f10d5fc7b6b7b7-partinfo-00000000-QINU UNIQe9f10d5fc7b6b7b7-partinfo-00000001-QINU