Difference between revisions of "Part:BBa K274003:Experience"
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E.coli turned dark green after transformation of this plasmid, it is not supposed to be so since there is no promoter upstream of the operon. We have not found the reason. | E.coli turned dark green after transformation of this plasmid, it is not supposed to be so since there is no promoter upstream of the operon. We have not found the reason. | ||
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| − | + | BBa_K274003 Review No.2 | |
| − | <I> | + | <I>UCL_London, Xiang Chen</I> |
|width='60%' valign='top'| | |width='60%' valign='top'| | ||
There is no XbaI restriction site in this plasmid, thus cannot be used to asseble after other parts. | There is no XbaI restriction site in this plasmid, thus cannot be used to asseble after other parts. | ||
| − | Diagnostic DNA gel was run to test all 4 restriction sites. BBa_K274003 was cut with XbaI, PstI, EcoRI & SpeI, XbaI & PstI. | + | Diagnostic DNA gel was run to test all 4 restriction sites. BBa_K274003 was cut with XbaI, PstI, EcoRI & SpeI, XbaI & PstI. A gel picture is shown as below. |
| + | [[Image:K274003 Diagnostic Gel .jpg|center|300px]] | ||
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| + | <partinfo>BBa_K274003 AddReview number </partinfo> | ||
| + | <I>Username</I> | ||
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Revision as of 23:04, 25 October 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K274003
User Reviews
E.coli turned dark green after transformation of this plasmid, it is not supposed to be so since there is no promoter upstream of the operon. We have not found the reason.
|
BBa_K274003 Review No.2 UCL_London, Xiang Chen |
There is no XbaI restriction site in this plasmid, thus cannot be used to asseble after other parts. Diagnostic DNA gel was run to test all 4 restriction sites. BBa_K274003 was cut with XbaI, PstI, EcoRI & SpeI, XbaI & PstI. A gel picture is shown as below.  |
UNIQd0c59fdc8c6d8279-partinfo-00000000-QINU UNIQd0c59fdc8c6d8279-partinfo-00000001-QINU