Difference between revisions of "Part:BBa K321003:Design"

(→‎Design Notes)
(→‎Design Notes)
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
A pre-existing PstI site was removed to meet the BioBrick standard.
+
A pre-existing PstI site was removed to meet the BioBrick standard.  A PCR product encoding the sequence was flanked with an XbaI site at the 5' and an SpeI site at the 3' for ligation with XbaI, SpeI double-digested pSB1C3 vector.
  
 
===Source===
 
===Source===

Revision as of 22:34, 25 October 2010

intracellular chain of CD3 zeta


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 274


Design Notes

A pre-existing PstI site was removed to meet the BioBrick standard. A PCR product encoding the sequence was flanked with an XbaI site at the 5' and an SpeI site at the 3' for ligation with XbaI, SpeI double-digested pSB1C3 vector.

Source

Cloned from the human genome.

References