Difference between revisions of "Part:BBa K302012:Experience"

Revision as of 21:57, 25 October 2010

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Applications of BBa_K302012

Graph1:

See below: Bacillus subtilis 168 cells (left),Bacillus subtilis expressing yneA(centre) Bacillus subtilis overexpressing yneA(right)

Graph 1 shows that overexpression of the yneA gene (ΔamyE:pSpac(hy)-oid::yneA) leads to a longer cell length compared with our control Bacillus subtilis 168.

Graph2:

See below: Bacillus subtilis 168 cells (left) and non-induced cells(right)

Graph 2 shows the percentage of cells at different lengths(μm)uninduced

Graph3:

See below: Bacillus subtilis 168 cells (left) and cells induced at 0.2mM IPTG(right)

Graph 3 shows the percentage of cells at different lengths(μm)induced at 0.2mM IPTG

Graph4:

See below: Bacillus subtilis 168 cells (left) and cells induced at 1mM IPTG(right)

Graph 4 shows the percentage of cells at different lengths(μm)induced at 1mM IPTG

Graphs 2,3 and 4 show a greater proportion of cells at a higher concentration of IPTG(1mM IPTG), compared with Bacillus subtilis 168 our control population.

User Reviews

UNIQdf6f272ef9b3928c-partinfo-00000000-QINU UNIQdf6f272ef9b3928c-partinfo-00000001-QINU

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 |[[Image:Teamnewcastle_yneA168.png|800px]]
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-|'''See below''': ''Bacillus subtilis 168'' cells (left) and ''Bacillus subtilis'' overexpressing ''yneA''(right)
+|'''See below''': ''Bacillus subtilis 168'' cells (left),''Bacillus subtilis'' expressing ''yneA''(centre) ''Bacillus subtilis'' overexpressing ''yneA''(right)
 |-
 |[[Image:Teamnewcastle_yneA168BS.jpg|300px]][[Image:Teamnewcastle_yneA1.jpg|300px]][[Image:Teamnewcastle_yneA.jpg|300px]]