Difference between revisions of "Part:BBa K302012:Experience"

(Applications of BBa_K302012)
(Applications of BBa_K302012)
Line 23: Line 23:
 
|'''Graph3''':
 
|'''Graph3''':
 
|-
 
|-
|[[Image:newcastle_0.2 induction.jpg|600px]][[Image:Teamnewcastle_0.2indBS.jpg|200px]]
+
|[[Image:newcastle_0.2 induction.jpg|600px]]
 
|-
 
|-
 
|'''See below''': ''Bacillus subtilis 168'' cells (left) and cells induced at 0.2mM IPTG(right)
 
|'''See below''': ''Bacillus subtilis 168'' cells (left) and cells induced at 0.2mM IPTG(right)
 
|-
 
|-
|[[Image:Teamnewcastle_yneA168BS.jpg|300px]][[Image:Teamnewcastle_noindBS.jpg|300px]]
+
|[[Image:Teamnewcastle_yneA168BS.jpg|300px]][[Image:Teamnewcastle_0.2indBS.jpg|300px]]
 
|-
 
|-
 
|Graph 3 shows the percentage of cells at different lengths(μm)induced at 0.2mM IPTG  
 
|Graph 3 shows the percentage of cells at different lengths(μm)induced at 0.2mM IPTG  

Revision as of 21:35, 25 October 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K302012

Graph1:
Teamnewcastle yneA168.png
Graph 1 shows that overexpression of the yneA gene (ΔamyE:pSpac(hy)-oid::yneA) leads to a longer cell length compared with our control Bacillus subtilis 168.
Graph2:
Newcastle no induction.jpg
See below: Bacillus subtilis 168 cells (left) and non-induced cells(right)
Teamnewcastle yneA168BS.jpgTeamnewcastle noindBS.jpg
Graph 2 shows the percentage of cells at different lengths(μm)uninduced
Graph3:
Newcastle 0.2 induction.jpg
See below: Bacillus subtilis 168 cells (left) and cells induced at 0.2mM IPTG(right)
Teamnewcastle yneA168BS.jpgTeamnewcastle 0.2indBS.jpg
Graph 3 shows the percentage of cells at different lengths(μm)induced at 0.2mM IPTG
Graph4:
Newcastle 1IPTG.jpgTeamnewcastle 1indBS.jpg
Graph 4 shows the percentage of cells at different lengths(μm)induced at 1mM IPTG

Graphs 2,3 and 4 show a greater proportion of cells at a higher concentration of IPTG(1mM IPTG), compared with Bacillus subtilis 168 our control population.

User Reviews

UNIQ478c1dd243058b79-partinfo-00000000-QINU UNIQ478c1dd243058b79-partinfo-00000001-QINU