Difference between revisions of "Part:BBa K302012:Experience"

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===Applications of BBa_K302012===
 
===Applications of BBa_K302012===
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[[Image:newcastle_no induction.jpg|600px]][[Image:newcastle_0.2 induction.jpg|600px]][[Image:newcastle_1IPTG.jpg|600px]]
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{|
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|'''Graph1''':
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|-
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|[[Image:Teamnewcastle_yneA168.png|800px]]
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|-
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|Graph 1 shows that overexpression of the ''yneA'' gene (Δ''amyE'':pSpac(hy)-oid::''yneA'')leads to a longer cell length compared with our wild type control ''Bacillus subtilis 168''.
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|-
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|'''Graph2''':
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|-
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|[[Image:newcastle_no induction.jpg|600px]]
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|-
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|Graph 2 shows the percentage of cells at different lengths(μm)uninduced
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|-
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|'''Graph3''':
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|-
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|[[Image:newcastle_0.2 induction.jpg|600px]]
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|-
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|Graph 3 shows the percentage of cells at different lengths(μm)induced at 0.2mM IPTG
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|-
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|'''Graph4''':
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|-
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|[[Image:newcastle_1IPTG.jpg|600px]]
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|-
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|Graph 4 shows the percentage of cells at different lengths(μm)induced at 1mM IPTG
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|}
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'''Graphs 2,3 and 4 show a greater proportion of cells at a higher concentration of IPTG(1mM IPTG), compared with ''Bacillus subtilis 168'' our control population. '''
  
 
===User Reviews===
 
===User Reviews===

Revision as of 21:04, 25 October 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K302012

Graph1:
Teamnewcastle yneA168.png
Graph 1 shows that overexpression of the yneA gene (ΔamyE:pSpac(hy)-oid::yneA)leads to a longer cell length compared with our wild type control Bacillus subtilis 168.
Graph2:
Newcastle no induction.jpg
Graph 2 shows the percentage of cells at different lengths(μm)uninduced
Graph3:
Newcastle 0.2 induction.jpg
Graph 3 shows the percentage of cells at different lengths(μm)induced at 0.2mM IPTG
Graph4:
Newcastle 1IPTG.jpg
Graph 4 shows the percentage of cells at different lengths(μm)induced at 1mM IPTG

Graphs 2,3 and 4 show a greater proportion of cells at a higher concentration of IPTG(1mM IPTG), compared with Bacillus subtilis 168 our control population.

User Reviews

UNIQa56fe77b1f253c14-partinfo-00000000-QINU UNIQa56fe77b1f253c14-partinfo-00000001-QINU