Difference between revisions of "Part:BBa K320011"

Revision as of 20:40, 25 October 2010

Origin for Asaia and resistance to Ampicillin

This BioBrick is constitued by an origin of replication for Asaia and E. coli (BBa_K32000) and a resistance to Ampicillin (BBa_P1002). It was initially designed to be inserted into (BBa_I51020). Warning: do not use this in Asaia, as Asaia is naturally resistant against Ampicillin, see our [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia website].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1166
Illegal AgeI site found at 1326
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 4

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K320011 short</partinfo>
-This BioBricks provide a resitance to Ampicilin and an origin of replication for Asaia or E.Coli.
+This BioBrick is constitued by an origin of replication for Asaia and E. coli (BBa_K32000) and a resistance to Ampicillin (<partinfo>BBa_P1002</partinfo>). It was initially designed to be inserted into (<partinfo>BBa_I51020</partinfo>).
+Warning: do not use this in Asaia, as Asaia is naturally resistant against Ampicillin, see our [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia website].
 <!-- Add more about the biology of this part here