Difference between revisions of "Part:BBa K323088:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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The 2010 iGEM team Slovenia used this part in combination with [[Part:BBa_K323089]] containing a corresponding DNA binding protein. Both parts were inserted into the low copy vector pSB4C5 ([[Part:pSB4C5]]) by tripoint ligation to form a '''universal testing device for DNA binding proteins'''.
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how you used this part and how it worked out.
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We have constructed 14 parts for testing of DNA binding proteins HivC ([[Part:BBa_K323090]] and [[Part:BBa_K323093]]), Gli1 ([[Part:BBa_K323092]] and [[Part:BBa_K323091]]), Zif268 ([[Part:BBa_K323094]], [[Part:BBa_K323097]] and [[Part:BBa_K323103]]), Jazz ([[Part:BBa_K323096]] and [[Part:BBa_K323095]]), Blues ([[Part:BBa_K323098]] and [[Part:BBa_K323101]]), PBSII ([[Part:BBa_K323100]] and [[Part:BBa_K323099]]) and TAL ([[Part:BBa_K323102]]).  
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Binding of the tested proteins was determined with the beta-galactosidase assay (for a detailed description of the method see the 2010 iGEM team Slovenia wiki). The representative results gathered below have been taken from different experiments and averaged to an arithmetic mean. The β-galactosidase activity of the culture without added arabinose, which should have indicated maximal activity due to minimal expression of the DNA binding protein, was normalized to 100 percent, and the β-galactosidase activity of the culture with added arabinose was compared to the latter.
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===Applications of BBa_K323088===
 
===Applications of BBa_K323088===

Revision as of 20:03, 25 October 2010

The 2010 iGEM team Slovenia used this part in combination with Part:BBa_K323089 containing a corresponding DNA binding protein. Both parts were inserted into the low copy vector pSB4C5 (Part:pSB4C5) by tripoint ligation to form a universal testing device for DNA binding proteins.

We have constructed 14 parts for testing of DNA binding proteins HivC (Part:BBa_K323090 and Part:BBa_K323093), Gli1 (Part:BBa_K323092 and Part:BBa_K323091), Zif268 (Part:BBa_K323094, Part:BBa_K323097 and Part:BBa_K323103), Jazz (Part:BBa_K323096 and Part:BBa_K323095), Blues (Part:BBa_K323098 and Part:BBa_K323101), PBSII (Part:BBa_K323100 and Part:BBa_K323099) and TAL (Part:BBa_K323102).

Binding of the tested proteins was determined with the beta-galactosidase assay (for a detailed description of the method see the 2010 iGEM team Slovenia wiki). The representative results gathered below have been taken from different experiments and averaged to an arithmetic mean. The β-galactosidase activity of the culture without added arabinose, which should have indicated maximal activity due to minimal expression of the DNA binding protein, was normalized to 100 percent, and the β-galactosidase activity of the culture with added arabinose was compared to the latter.



Applications of BBa_K323088

User Reviews

UNIQ2843194592e3dee8-partinfo-00000000-QINU UNIQ2843194592e3dee8-partinfo-00000001-QINU