Difference between revisions of "Part:BBa K415300"

Revision as of 19:50, 25 October 2010

Collins UV Toggle - Ptrc:cI:tt:Plambda:lacI:tt

From Programmable cells: Interfacing natural and engineered gene networks, PNAS.

The original pTSMa plasmid from the Collins paper. The parts have since been biobricked into pSB1C3.

The [http://www.nature.com/nature/journal/v403/n6767/abs/403339a0.html|Collins UV Toggle] (2000) biobricked. It is a bistable toggle (on or off state) and switching to state 1 is induced by UV exposure and to state 2 is IPTG. If the toggle is set with IPTG, the cells will express cI which will inhibit Plambda. If this is exposed to certain levels of UV (see power modulations) cI is cleaved by Rec-A (a UV induced enzyme) and lacI expression begins and inhibits Ptrc.

UV Power Modulation
Photos	Image
FACS	Image

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1773
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K415300 short</partinfo>
-From <i>Construction of a genetic toggle switch in E. Coli.</i> Collins Toggle, Advanced Biotechnology, BU.[[Image:CollinsToggle.png|frame|150px|right|The original pTSMa plasmid from the Collins paper. The parts have since been biobricked into pSB1C3.]]
+From <i>Programmable cells: Interfacing natural and engineered gene networks</i>, PNAS. [[Image:CollinsToggle.png|frame|150px|right|The original pTSMa plasmid from the Collins paper. The parts have since been biobricked into pSB1C3.]]
 The [http://www.nature.com/nature/journal/v403/n6767/abs/403339a0.html|Collins UV Toggle] (2000) biobricked. It is a bistable toggle (on or off state) and switching to state 1 is induced by UV exposure and to state 2 is IPTG. If the toggle is set with IPTG, the cells will express cI which will inhibit Plambda. If this is exposed to certain levels of UV (see power modulations) cI is cleaved by Rec-A (a UV induced enzyme) and lacI expression begins and inhibits Ptrc.