Difference between revisions of "Part:BBa R0084"
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Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompF. Phosphorylated OmpR binds to the three operator sites and activates transcription. | Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompF. Phosphorylated OmpR binds to the three operator sites and activates transcription. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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In nature, this promoter is upstream of the ompF porin gene and is both activating and repressing. The regulation of ompF is determined by the EnvZ-OmpR osmosensing machinery. EnvZ phosphorylates OmpR to OmpR-P. At low osmolarity, EnvZ has a low activity, producing a small concentration of OmpR-P. This OmpR-P binds to high-affinity sites on ompF, activating transcription. At high osmolarity, however, EnvZ is more active, creating a high OmpR-P concentration. OmpR-P then binds to the low-affinity sites on ompF, repressing transcription. See Notes section for further discussion of negative regulation. | In nature, this promoter is upstream of the ompF porin gene and is both activating and repressing. The regulation of ompF is determined by the EnvZ-OmpR osmosensing machinery. EnvZ phosphorylates OmpR to OmpR-P. At low osmolarity, EnvZ has a low activity, producing a small concentration of OmpR-P. This OmpR-P binds to high-affinity sites on ompF, activating transcription. At high osmolarity, however, EnvZ is more active, creating a high OmpR-P concentration. OmpR-P then binds to the low-affinity sites on ompF, repressing transcription. See Notes section for further discussion of negative regulation. | ||
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<partinfo>BBa_R0084 SequenceAndFeatures</partinfo> | <partinfo>BBa_R0084 SequenceAndFeatures</partinfo> | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_R0084 parameters</partinfo> | <partinfo>BBa_R0084 parameters</partinfo> | ||
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Revision as of 00:59, 5 June 2006
Promoter (OmpR, positive)
Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompF. Phosphorylated OmpR binds to the three operator sites and activates transcription.
Usage and Biology
In nature, this promoter is upstream of the ompF porin gene and is both activating and repressing. The regulation of ompF is determined by the EnvZ-OmpR osmosensing machinery. EnvZ phosphorylates OmpR to OmpR-P. At low osmolarity, EnvZ has a low activity, producing a small concentration of OmpR-P. This OmpR-P binds to high-affinity sites on ompF, activating transcription. At high osmolarity, however, EnvZ is more active, creating a high OmpR-P concentration. OmpR-P then binds to the low-affinity sites on ompF, repressing transcription. See Notes section for further discussion of negative regulation.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]