Difference between revisions of "Part:BBa K364319"
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Our Gal4 is flanked by RFC 25 Prefix and Suffix, and thus can be used for assembly of in frame composite parts. | Our Gal4 is flanked by RFC 25 Prefix and Suffix, and thus can be used for assembly of in frame composite parts. | ||
| − | [[Image:Team Debrecen pSB1C3-Gal4.jpg|800px|thumb|center|Picture of gel electrophoresis: Gal4 in pSB1C3 | + | [[Image:Team Debrecen pSB1C3-Gal4.jpg|800px|thumb|center|Picture of gel electrophoresis: Gal4 in pSB1C3 results a 441 bp long insert.]] |
[[Image:Gal4.jpg]] | [[Image:Gal4.jpg]] | ||
Revision as of 18:05, 25 October 2010
Gal4 DBD
This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. This protein contains a fungal Zn(2)-Cys(6) binuclear cluster domain.
Our Gal4 is flanked by RFC 25 Prefix and Suffix, and thus can be used for assembly of in frame composite parts.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 218
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 137