Difference between revisions of "Part:BBa R1062"
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The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription. | The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Medium strength promoter in the absense of LuxR/HSL. May not be well matched with other '10 series' promoters. [jb 5/24/04] | Medium strength promoter in the absense of LuxR/HSL. May not be well matched with other '10 series' promoters. [jb 5/24/04] | ||
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<partinfo>BBa_R1062 SequenceAndFeatures</partinfo> | <partinfo>BBa_R1062 SequenceAndFeatures</partinfo> | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_R1062 parameters</partinfo> | <partinfo>BBa_R1062 parameters</partinfo> | ||
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Revision as of 00:59, 5 June 2006
Promoter, Standard (luxR and HSL regulated -- lux pR)
[Note: This is the same part as R0062 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001].
Promoter activated by LuxR in concert with HSL
The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
Usage and Biology
Medium strength promoter in the absense of LuxR/HSL. May not be well matched with other '10 series' promoters. [jb 5/24/04]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]