Difference between revisions of "Part:BBa K364333"
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Expression vector from standard BioBrick parts, expressing Gal4 - Ecdysone receptor LBD composite part.
 
Expression vector from standard BioBrick parts, expressing Gal4 - Ecdysone receptor LBD composite part.
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The Gal4-EcR Chimeric nuclear receptor is an artificial eukaryotic TF made of Gal4 DBD (DNA Binding Domain) and D. Melanogaster nuclear hormone receptor LBD (Ligand Binding Domain) which can be expressed by this expression vector.
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The minimal CMV promoter ensures a continuous expression of the chimeric construct, but furthermore it can be expressed in an inducible form using the Tetracyclin-controlled transcriptional activation system.
  
 
[[Image:Team Debrecen pSB1A3-TRE-Gal4-EcR-PolyA.jpg|800px|thumb|center|Picture of gel electrophoresis: TRE-Gal4-EcR-PolyA in pSB1A3 results a new expression vector from standard Biobrick parts. The TRE-Gal4-EcR LBD-PolyA insert is 1589 bp long, without polyA which was inserted in the last step is 191 bp shorter.]]
 
[[Image:Team Debrecen pSB1A3-TRE-Gal4-EcR-PolyA.jpg|800px|thumb|center|Picture of gel electrophoresis: TRE-Gal4-EcR-PolyA in pSB1A3 results a new expression vector from standard Biobrick parts. The TRE-Gal4-EcR LBD-PolyA insert is 1589 bp long, without polyA which was inserted in the last step is 191 bp shorter.]]

Revision as of 16:21, 25 October 2010

TRE-Gal4-EcR-PolyA in PSB1A3 (expression vector)

Expression vector from standard BioBrick parts, expressing Gal4 - Ecdysone receptor LBD composite part.

The Gal4-EcR Chimeric nuclear receptor is an artificial eukaryotic TF made of Gal4 DBD (DNA Binding Domain) and D. Melanogaster nuclear hormone receptor LBD (Ligand Binding Domain) which can be expressed by this expression vector.

The minimal CMV promoter ensures a continuous expression of the chimeric construct, but furthermore it can be expressed in an inducible form using the Tetracyclin-controlled transcriptional activation system.

Picture of gel electrophoresis: TRE-Gal4-EcR-PolyA in pSB1A3 results a new expression vector from standard Biobrick parts. The TRE-Gal4-EcR LBD-PolyA insert is 1589 bp long, without polyA which was inserted in the last step is 191 bp shorter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1461
    Illegal XhoI site found at 547
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 466