Difference between revisions of "Part:BBa K325905"

Latest revision as of 16:15, 25 October 2010

Bacterial Lux reporter
CDEG pλ AB
(E. coli optimised)

This part contains two operons. The first consists of the LuxCDEG genes from V.fischeri that have been codon optimised for use in E.coli. These genes produce the substrate for the LuxAB genes encoded by the second reading frame. The LuxAB genes have been derived from Xenorhabdus luminescens. The first operon can be placed under any promoter to achieve inducible or constitutive light output. The second operon is controlled by the strong constitutive pLambda promoter.

As the proteins of the V.fischeri Lux operon are easily damaged by a high incubation temperature, transformed cells should not be grown at more than 30°C if light production is desired.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 3112
Illegal AgeI site found at 3327
Illegal AgeI site found at 3915
Illegal AgeI site found at 4898
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 5417

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 __NOTOC__
 <partinfo>BBa_K325905 short</partinfo>
-a
+This part contains two operons. The first consists of the LuxCDEG genes from V.fischeri that have been codon optimised for use in E.coli. These genes produce the substrate for the LuxAB genes encoded by the second reading frame. The LuxAB genes have been derived from Xenorhabdus luminescens. The first operon can be placed under any promoter to achieve inducible or constitutive light output. The second operon is controlled by the strong constitutive pLambda promoter.
+As the proteins of the V.fischeri Lux operon are easily damaged by a high incubation temperature, transformed cells should not be grown at more than 30°C if light production is desired.
 <!-- Add more about the biology of this part here