Difference between revisions of "Part:BBa K364333"

Revision as of 15:50, 25 October 2010

TRE-Gal4-EcR-PolyA in PSB1A3 (expression vector)

Expression vector from standard BioBrick parts, expressing Gal4 - Ecdysone receptor LBD composite part.

Picture of gel electrophoresis: TRE-Gal4-EcR-PolyA in pSB1A3 results a new expression vector from standard Biobrick parts. The TRE-Gal4-EcR LBD-PolyA insert is 1589 bp long, without polyA which was inserted in the last step is 191 bp shorter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1461
Illegal XhoI site found at 547
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 466

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K364333 short</partinfo>
-Expression vector from standard BioBrick parts, epressing Gal4 - Ecdysone receptor LBD composite part.
+Expression vector from standard BioBrick parts, expressing Gal4 - Ecdysone receptor LBD composite part.
 [[Image:Team Debrecen pSB1A3-TRE-Gal4-EcR-PolyA.jpg|800px|thumb|center|Picture of gel electrophoresis: TRE-Gal4-EcR-PolyA in pSB1A3 results a new expression vector from standard Biobrick parts. The TRE-Gal4-EcR LBD-PolyA insert is 1589 bp long, without polyA which was inserted in the last step is 191 bp shorter.]]