Difference between revisions of "Part:BBa K322451"

 
 
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<partinfo>BBa_K322451 short</partinfo>
 
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Mutations Ile423Leu Asp436Gly and Leu530Arg increase the bioluminescence intensity by a factor of 12.5 according to Fujii et al. (2007). It is codon optimised for expression in E. coli.  
 
Mutations Ile423Leu Asp436Gly and Leu530Arg increase the bioluminescence intensity by a factor of 12.5 according to Fujii et al. (2007). It is codon optimised for expression in E. coli.  
  
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Reference
 
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Fujii, H., Noda, K., Asami, Y., Kuroda, A., Sakata, M. & Tokida, A. (2007). Increase in
 
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bioluminescence intensity of firefly luciferase using genetic modification. Analytical
 
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Biochemistry 366, 131-136.
 
  
 
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Latest revision as of 14:39, 25 October 2010

Photinus pyralis luciferase mutated for increased bioluminescence.

Mutations Ile423Leu Asp436Gly and Leu530Arg increase the bioluminescence intensity by a factor of 12.5 according to Fujii et al. (2007). It is codon optimised for expression in E. coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1281
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1417
    Illegal NgoMIV site found at 1458
    Illegal AgeI site found at 1150
  • 1000
    COMPATIBLE WITH RFC[1000]