Difference between revisions of "Part:BBa R0063"
| Line 4: | Line 4: | ||
The lux cassette of V. fischeri contains a left and a right promoter. The left promoter gives weak constitutive expression of downstream genes.This expression is down-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription from Pr, repressing transcription from Pl | The lux cassette of V. fischeri contains a left and a right promoter. The left promoter gives weak constitutive expression of downstream genes.This expression is down-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription from Pr, repressing transcription from Pl | ||
| + | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | |||
Medium strength promoter in the absence of LuxR/HSL. [jb, 5/24/04] | Medium strength promoter in the absence of LuxR/HSL. [jb, 5/24/04] | ||
| Line 11: | Line 13: | ||
<partinfo>BBa_R0063 SequenceAndFeatures</partinfo> | <partinfo>BBa_R0063 SequenceAndFeatures</partinfo> | ||
| + | |||
| + | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_R0063 parameters</partinfo> | <partinfo>BBa_R0063 parameters</partinfo> | ||
| + | <!-- --> | ||
Revision as of 00:59, 5 June 2006
Promoter (luxR & HSL regulated -- lux pL)
The lux cassette of V. fischeri contains a left and a right promoter. The left promoter gives weak constitutive expression of downstream genes.This expression is down-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription from Pr, repressing transcription from Pl
Usage and Biology
Medium strength promoter in the absence of LuxR/HSL. [jb, 5/24/04]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]