Difference between revisions of "Part:BBa K342002:Design"

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  <a href="http://2010.igem.org/Team:INSA-Lyon/Project/References#et1"><font color= black><i>Banki et al.</i><font>
 
  <a href="http://2010.igem.org/Team:INSA-Lyon/Project/References#et1"><font color= black><i>Banki et al.</i><font>
 
</a></p>
 
</a></p>
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<p>The phasin sequence is the same as the biobrick <a ref=https://parts.igem.org/Part:BBa_K208001><font color= black><i><u>BBa_K208001</u></i></font></a> from the Utah 2009 team.  
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<p>The phasin sequence is the same as the biobrick <a ref="https://parts.igem.org/Part:BBa_K208001"><font color= "black"><i><u>BBa_K208001</u></i></font></a> from the Utah 2009 team.  
 
<p>The sequencing of this part is difficult because of the repetition of phasin's sequence.</p>
 
<p>The sequencing of this part is difficult because of the repetition of phasin's sequence.</p>
 
</html>
 
</html>

Revision as of 14:14, 25 October 2010

Phasin-Phasin Intein (for silver standard fusion)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1693
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1279
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has been designed thanks to the results of the publication Banki et al.

The phasin sequence is the same as the biobrick BBa_K208001 from the Utah 2009 team.

The sequencing of this part is difficult because of the repetition of phasin's sequence.

Source

Ligation of two synthetized parts : Phasin and Phasin-Intein

References