Difference between revisions of "Part:BBa K395301"

Revision as of 14:02, 25 October 2010

PompC(C) Promoter regulated by phosphorylated OmpR

This part is positively regulated by phosphorylated OmpR, a response regulator in EnvZ-OmpR two-component system. The EnvZ-OmpR system of E. coli regulates the transcriptional activity of this promoter in response to change in extracellular osmolarity. This promoter is a derivatives of the upstream of POmpC(WT)BBa_R0082 which originally comprises 3 distinct phosphorylated OmpR recognition sites, binding site 1, binding site 2 and binding site 3. OmpC(C) promoter consists of binding site 1 and additional 4 base pairs before the canonical -35 and -10 region. [http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC ...see more about PompC series]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 24
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K395301 short</partinfo>
-This part is regulated by phosphorylated OmpR, a response regulator in EnvZ-OmpR two-component system. The EnvZ-OmpR system of E. coli regulates the transcriptional activity of this promoter in response to change in extracellular osmolarity. This promoter is a derivatives of P''OmpC(WT)''BBa_R0082 consists of binding site 1 and additional 4 base pairs before the canonical -35 and -10 region. Originally, the wild-type promoter comprises 3 distinct phosphorylated OmpR recognition sites which are binding site 1, binding site 2 and binding site 3. [http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC ...see more about P''ompC'' series]
+This part is positively regulated by phosphorylated OmpR, a response regulator in EnvZ-OmpR two-component system. The EnvZ-OmpR system of E. coli regulates the transcriptional activity of this promoter in response to change in extracellular osmolarity. This promoter is a derivatives of the upstream of P''OmpC(WT)''BBa_R0082 which originally comprises 3 distinct phosphorylated OmpR recognition sites, binding site 1, binding site 2 and binding site 3. ''OmpC(C)'' promoter consists of binding site 1 and additional 4 base pairs before the canonical -35 and -10 region. [http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC ...see more about P''ompC'' series]
   <!-- Add more about the biology of this part here.