Difference between revisions of "Part:BBa K389015:Experience"

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Revision as of 12:55, 25 October 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K389015

Response time

The system needs at least one hour to show a measurable reaction to an induction with acetosyringone. In the following illustration the reaction of the system to induction with 200 µM acetosyringone in the exponential growth phase is shown. For a good separation of the induced system from the uninduced system at least two hours are needed.

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Cultivation of BBa_K389015 in Escherichia coli DB3.1. Induction with 200 µM acetosyringone during the exponential growth phase.


Data Analysis

Because the luciferase accumulation is very different in different cultivations, the uninduced negative control was used as internal standard. To show the behaviour of the VirA/G signaling system when induced, the ratio ɸI between induced (i) and uninduced (u) relative luminescence unit (RLU) per OD600 is calculated:


Bielefeld Quotient RLU.jpg
(1)


As seen above, at least one hour is needed to separate the induced luminescence signal from the uninduced, so ɸI > 1.

Protocols

1. bla


User Reviews

UNIQ2e386af5ff812e71-partinfo-00000001-QINU UNIQ2e386af5ff812e71-partinfo-00000002-QINU