Difference between revisions of "Part:BBa K358023"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K358023 short</partinfo>
 
<partinfo>BBa_K358023 short</partinfo>
  
 
This part is the same to BBa_K358020[Lysisbox], except for its constitutive promoter[BBa_J23100].
 
This part is the same to BBa_K358020[Lysisbox], except for its constitutive promoter[BBa_J23100].
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So, this plasmid induces lambda lysis cassette more than BBa_K358020.
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So, this plasmid is able to induce the killer gene more than the plasmid contains BBa_K358020.
  
 
This part also contains S&#916;TMD1, anti-killergene, regulated by lacP.
 
This part also contains S&#916;TMD1, anti-killergene, regulated by lacP.
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When transform this part, you must induce the S&#916;TMD1 highly, do not repress lacP, so that the cell lysis will be prevented.
 
When transform this part, you must induce the S&#916;TMD1 highly, do not repress lacP, so that the cell lysis will be prevented.
  
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This is a long part and a lactose operon was deleted unexpectedly in our experiments.  So, we recomend you that it is better to set the cultural temperature at 30C and store this part as a plasmid, not a glycerole stock.
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This is a long part and a lactose operon was deleted sometimes unexpectedly in our experiments.  So, we recomend you that it is better to set the cultural temperature at 30C and store this part as a plasmid, not a glycerole stock.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 10:27, 25 October 2010

Lysisbox -type1- ver.2

This part is the same to BBa_K358020[Lysisbox], except for its constitutive promoter[BBa_J23100]. So, this plasmid is able to induce the killer gene more than the plasmid contains BBa_K358020.

This part also contains SΔTMD1, anti-killergene, regulated by lacP.

When transform this part, you must induce the SΔTMD1 highly, do not repress lacP, so that the cell lysis will be prevented.

This is a long part and a lactose operon was deleted sometimes unexpectedly in our experiments. So, we recomend you that it is better to set the cultural temperature at 30C and store this part as a plasmid, not a glycerole stock.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1253
    Illegal NheI site found at 1276
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1025