Difference between revisions of "Part:BBa K422011:Design"
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. MreB-C is an A-part compatible for N-Terminal fusion. For more information see [http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning].
+
BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. MreB-C is an C-part compatible for N-Terminal fusion. For more information see [http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning].
  
 
===Source===
 
===Source===

Revision as of 08:52, 25 October 2010

mreB (AarI C-part)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 170
    Illegal PstI site found at 790
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 170
    Illegal PstI site found at 790
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 170
    Illegal PstI site found at 790
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 170
    Illegal PstI site found at 790
    Illegal AgeI site found at 487
    Illegal AgeI site found at 805
    Illegal AgeI site found at 892
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. MreB-C is an C-part compatible for N-Terminal fusion. For more information see [http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning].

Source

Escherichia coli genome

References

http://2010.igem.org/Team:ETHZ_Basel

Kruse, Bork-Jensen and Gerdes: The morphogenetic MreBCD proteins of Escherichia coli form an essential membrane-bound complex. Molecular Microbiology. 2005;55:1.