Difference between revisions of "Part:BBa K422010"

Revision as of 08:49, 25 October 2010

trig (AarI A-part)

Biological Background

The ribosome binding domain of the trigger factor trigA binds to the large subunit of the ribosome [1].

Cloning strategy

BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. CheB-A is an A-part compatible for N-Terminal fusion. For more information see [http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning].

References

[1] Hesterkamp, Deuerling and Bukau: The Amino-terminal 118 amino acids of Escherichia coli Trigger factor constitute a domain that is necessary and sufficient for binding to ribosomes. The Journal of Biologcial Chemistry. 1997; 272:35.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 364
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 364
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 364
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 364
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 88

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K422010 short</partinfo>
-xxx
+===Biological Background===
+The ribosome binding domain of the trigger factor trigA binds to the large subunit of the ribosome [1].
+===Cloning strategy===
+BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. '''CheB-A''' is an A-part compatible for N-Terminal fusion. For more information see [http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning].
+===References===
+[1] Hesterkamp, Deuerling and Bukau: The Amino-terminal 118 amino acids of Escherichia coli Trigger factor constitute a domain that is necessary and sufficient for binding to ribosomes. The Journal of Biologcial Chemistry. 1997; 272:35.
 <!-- Add more about the biology of this part here