Difference between revisions of "Part:BBa K415148:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K415148=== | ===Applications of BBa_K415148=== | ||
| + | '''Assessing p8-Jun incorporation into phage coat''' | ||
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| + | We transformed XL1-Blue cells with BBa_K415149, grew cells up without induction and infected with M13K07 helper phage. After 5 hours, the phage was purified, denatured, and western blotted with an anti-HA antibody. | ||
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| + | There was no signal from the blot, indicating that inducing the part may be necessary for incorporation into the phage coat. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 06:06, 25 October 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K415148
Assessing p8-Jun incorporation into phage coat
We transformed XL1-Blue cells with BBa_K415149, grew cells up without induction and infected with M13K07 helper phage. After 5 hours, the phage was purified, denatured, and western blotted with an anti-HA antibody.
There was no signal from the blot, indicating that inducing the part may be necessary for incorporation into the phage coat.
User Reviews
UNIQ786711f3e75051e5-partinfo-00000000-QINU UNIQ786711f3e75051e5-partinfo-00000001-QINU