Difference between revisions of "Part:BBa K346004"

Revision as of 01:42, 25 October 2010

RBS(B0034)_MBP(lead metal binding peptide egineered from PbrR)+Terminator(B0015)

This part consists of an RBS, a mbp which codes for the lead binding peptide and a terminator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K346004 short</partinfo>
-This part is combined with a rbs, a mbp which codes for the metal binding peptide and a terminator.
+This part consists of  an RBS, a mbp which codes for the lead binding peptide and a terminator.
 <!-- Add more about the biology of this part here
-===Usage and Biology===
+===Description===
+MerR family TFs share a high similarity at the C-terminal metal binding domain , which indicates a similar metal recognition mechanism and metal-protein complex structure.Previous work shows that C-terminal metal binding domain can act as a metal accumulator without the help of the N-terminal DNA binding domain.Our work also show that the engineered MBP which is constructed from MerR can work properly. As a consequence, the lead Metal Binding Peptide engineered from PbrR, one member of the MerR family, can be designed and constructed in the similar way as the mercury MBP does.In our project, we try to tandem two metal binding domains together to make a high performance and less energy consuming metal binding peptide.This part works as the core part in the lead bioabsorption device.
+[[Image:PbrR.jpg]]       [[Image:lead PbrR1.jpg]]
+The figure shows the structure of PbrR and MBP(lead). The left figure is PbrR, the lead-responsive transcription factor,one member of MerR family. The right figure shows the predicted structure of resulted metal binding peptide of lead. lead ions are indicated as black balls in metal binding pockets.
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