Difference between revisions of "Part:BBa K323043"

Revision as of 20:46, 24 October 2010

DNA program 12346

This part contains a DNA program - a specifically designed nucleotide sequence containing zinc finger binding sites. The program was designed in such a way, that chimeric enzymes (fused with zinc finger proteins) for synthesis of violacein bind to the sequence in the order required to produce the final product. The part contains binding sites for zinc finger proteins in the following order: Jazz - Blues - Zif268 - PBSII - Gli1.

We have bound four out of five chimeric enzymes (Blues_VioA, Zif268_VioB, PBSII_VioE and HivC_VioD), encoded by Part:BBa_K323135 and Part:BBa_K323132, to the program sequence and have shown that the order of the enzymes bound on the DNA program determines the final product.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 16:25, 24 October 2010 (view source) JernejT (Talk \| contribs) ← Older edit		Revision as of 20:46, 24 October 2010 (view source) TinaL (Talk \| contribs) Newer edit →
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	This part contains a DNA program - a specifically designed nucleotide sequence containing zinc finger binding sites. The program was designed in such a way, that chimeric enzymes (fused with zinc finger proteins) for synthesis of violacein bind to the sequence in the order required to produce the final product. The part contains binding sites for zinc finger proteins in the following order: Jazz - Blues - Zif268 - PBSII - Gli1.		This part contains a DNA program - a specifically designed nucleotide sequence containing zinc finger binding sites. The program was designed in such a way, that chimeric enzymes (fused with zinc finger proteins) for synthesis of violacein bind to the sequence in the order required to produce the final product. The part contains binding sites for zinc finger proteins in the following order: Jazz - Blues - Zif268 - PBSII - Gli1.

−	We have bound four out of five chimeric enzymes (Blues_VioA, Zif268_VioB, PBSII_VioE and HivC_VioD), encoded by [[Part:BBa_K323135]] and [[Part:BBa_K323132]], to the program sequence and have shown that the order of the enzymes bound on the DNA program determines the final product. In our case, we have produced large amounts of deoxyviolacein rather than violacein, which would have been produced if the five enzymes were randomly distributed in the cell or bound to a DNA program with all five zinc figer binding sites.	+	We have bound four out of five chimeric enzymes (Blues_VioA, Zif268_VioB, PBSII_VioE and HivC_VioD), encoded by [[Part:BBa_K323135]] and [[Part:BBa_K323132]], to the program sequence and have shown that the order of the enzymes bound on the DNA program determines the final product.