Difference between revisions of "Part:BBa K364316:Design"

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===Design Notes===
 
===Design Notes===
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The construction of the LBD and Backbone will be done with RFC 25.
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The construction of the LBD and Backbone have been done with RFC 25.
  
 
===Source===
 
===Source===

Revision as of 18:40, 24 October 2010

NHR-25 LBD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 419
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The construction of the LBD and Backbone have been done with RFC 25.

Source

C. elegans orphan nuclear receptor. Artifically synthetized Ligand Binding Domain of C. elegans NHR-25 placed into pSB1C3 carrier vector.

References

1. Brooks DR, Appleford PJ, Murray L, Isaac RE. "An essential role in molting and morphogenesis of Caenorhabditis elegans for ACN-1, a novel member of the angiotensin-converting enzyme family that lacks a metallopeptidase active site." [http://www.jbc.org/content/278/52/52340.long2003 J Biol Chem. Dec 26; Epub 2003 Oct 14.]