Difference between revisions of "Part:BBa B0012:Design"
 
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<partinfo>BBa_B0012 short</partinfo>
 
<partinfo>BBa_B0012 short</partinfo>
  
 
<partinfo>BBa_B0012 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_B0012 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The      5' end cutoff was placed immediately after the TAA stop codon and the 3' end      cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).
 
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The      5' end cutoff was placed immediately after the TAA stop codon and the 3' end      cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).
  
 
Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
 
Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
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===Source===
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Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4      [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Search&db=nucleotide&term=V01146 [Genbank V01146] ].
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===References===

Latest revision as of 00:49, 5 June 2006


TE from coliphageT7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).

Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.



Source

Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Search&db=nucleotide&term=V01146 [Genbank V01146] ].

References