Difference between revisions of "Part:BBa K364202:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K364202=== | ===Applications of BBa_K364202=== | ||
| + | In our system we would like to induce apoptosis by increasing the amount of proapoptotic proteins such as BAX by producing an expression vector containing TRE-minimal CMV promoter, BAX or PUMA and polyA tail. The proapoptotic proteins are able to express only in a controlled form using the advanced Tet-On expression system. The overexpression of these proteins can lead to Mitochondrial Outer Membrane Permeabilisation (MOMP) which is a point of no return in the mitochondrial pathway of Programmed Cell Death (PCD). The goal is to “switch off” the cells, which were genetically modified by us, after they “finished their work” in the recent local environment combining the components of the tetracycline-inducible system in self-contained retroviral and plasmid vectors. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 13:19, 24 October 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K364202
In our system we would like to induce apoptosis by increasing the amount of proapoptotic proteins such as BAX by producing an expression vector containing TRE-minimal CMV promoter, BAX or PUMA and polyA tail. The proapoptotic proteins are able to express only in a controlled form using the advanced Tet-On expression system. The overexpression of these proteins can lead to Mitochondrial Outer Membrane Permeabilisation (MOMP) which is a point of no return in the mitochondrial pathway of Programmed Cell Death (PCD). The goal is to “switch off” the cells, which were genetically modified by us, after they “finished their work” in the recent local environment combining the components of the tetracycline-inducible system in self-contained retroviral and plasmid vectors.
User Reviews
UNIQ62d5d69ab0332607-partinfo-00000000-QINU UNIQ62d5d69ab0332607-partinfo-00000001-QINU