Difference between revisions of "Part:BBa K346037"
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The T3 polymerase is also a specific activator of a series of T3 promoter that can regulate downstream genes.We designed this polymerase to be a system that is parallel to T7 polymerase driven expression. | The T3 polymerase is also a specific activator of a series of T3 promoter that can regulate downstream genes.We designed this polymerase to be a system that is parallel to T7 polymerase driven expression. | ||
The amplifier is constructed by engineering PhiR73 and Po promoter together. PhiR73 is a phage derived activator that can activate Po promoter. We put antigen 43 downstream of Po promoter so that it will be driven indirectly by the expression of PhiR73. We then put PhiR73 under the regulation of T3 promoter, so that antigen 43 gene is now controlled by T3 promoter. In this way we have engineered a inductive expression part. | The amplifier is constructed by engineering PhiR73 and Po promoter together. PhiR73 is a phage derived activator that can activate Po promoter. We put antigen 43 downstream of Po promoter so that it will be driven indirectly by the expression of PhiR73. We then put PhiR73 under the regulation of T3 promoter, so that antigen 43 gene is now controlled by T3 promoter. In this way we have engineered a inductive expression part. | ||
| − | + | [[Image:phir+po+agn.jpg]] | |
| + | Figure 1: the clone plasmid that can achieve the inductive expression of antigen 43. Green circle indicate the ribosome binding sites. Red hexagon indicates the terminator. PhiR73 and Po promoter are used as a delay and amplify module. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 11:29, 24 October 2010
T3 promoter drived amplifier+Agn43
This part can generate antigen 43 that can cause auto-aggregation under the regulation of T3 RNA polymerase.
The T3 polymerase is also a specific activator of a series of T3 promoter that can regulate downstream genes.We designed this polymerase to be a system that is parallel to T7 polymerase driven expression.
The amplifier is constructed by engineering PhiR73 and Po promoter together. PhiR73 is a phage derived activator that can activate Po promoter. We put antigen 43 downstream of Po promoter so that it will be driven indirectly by the expression of PhiR73. We then put PhiR73 under the regulation of T3 promoter, so that antigen 43 gene is now controlled by T3 promoter. In this way we have engineered a inductive expression part.
Figure 1: the clone plasmid that can achieve the inductive expression of antigen 43. Green circle indicate the ribosome binding sites. Red hexagon indicates the terminator. PhiR73 and Po promoter are used as a delay and amplify module.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 709
Illegal NgoMIV site found at 1933
Illegal NgoMIV site found at 2443
Illegal NgoMIV site found at 2464
Illegal AgeI site found at 1459
Illegal AgeI site found at 2203
Illegal AgeI site found at 2617
Illegal AgeI site found at 2859 - 1000COMPATIBLE WITH RFC[1000]