Difference between revisions of "Part:BBa K346038"

Latest revision as of 11:25, 24 October 2010

PmerT promoter+T3 polymerase

PmerT promoter is activated by intracellular Hg(II).When merR dimer bind Hg(II) in the cytosol, it will bind to PmerT and activate it, so the gene downstream will be expressed. T3 RNA polymerase can regulate downstream T3 promoter, which activate target gene's expression.By constructing these two parts together, we are able to regulate and express gene under T3 promoter, and this expression is indirectly regulated by intracellular Hg(II).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2650

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 __NOTOC__
 <partinfo>BBa_K346038 short</partinfo>
-PmerT promoter is activated by intracellular Hg(II). T3 RNA polymerase can regulate downstream T3 promoter, which activate target gene's expression.
+PmerT promoter is activated by intracellular Hg(II).When merR dimer bind Hg(II) in the cytosol, it will bind to PmerT and activate it, so the gene downstream will be expressed.
+T3 RNA polymerase can regulate downstream T3 promoter, which activate target gene's expression.By constructing these two parts together, we are able to regulate and express gene under T3 promoter, and this expression is indirectly regulated by intracellular Hg(II).
 <!-- Add more about the biology of this part here