Difference between revisions of "Part:BBa K313002"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K313002 short</partinfo>
 
<partinfo>BBa_K313002 short</partinfo>
  
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Flpe is a site-specific DNA recombinase that that recognizes FRT sequence.
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Flpe is a site-specific DNA recombinase that recognizes FRT sequence.
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Flpe was derived from yeaset site-specific DNA recombinase Flp.
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Flpe was derived from yeast site-specific DNA recombinase Flp.
 
(Buchholz, et al.,Improved properties of FLP recombinase evolved by cycling mutagenesis. Nature. Biotechnology, 16:657-662 ( 1998 ))
 
(Buchholz, et al.,Improved properties of FLP recombinase evolved by cycling mutagenesis. Nature. Biotechnology, 16:657-662 ( 1998 ))
  
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It's internal EcoRI and SpeI site has been removed using site-directed mutagenesis.
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We successfully removed the internal EcoRI and SpeI sites using site-directed mutagenesis.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 06:57, 24 October 2010

flpe DNA recombinase

Flpe is a site-specific DNA recombinase that recognizes FRT sequence. Flpe was derived from yeast site-specific DNA recombinase Flp. (Buchholz, et al.,Improved properties of FLP recombinase evolved by cycling mutagenesis. Nature. Biotechnology, 16:657-662 ( 1998 ))

We successfully removed the internal EcoRI and SpeI sites using site-directed mutagenesis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 504
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]