Difference between revisions of "Part:BBa K323086"
| Line 2: | Line 2: | ||
<partinfo>BBa_K323086 short</partinfo> | <partinfo>BBa_K323086 short</partinfo> | ||
| − | This part is composited from the Gli1 zinc finger gene([[Part:BBa_K165007]]) and the ''vio''C gene, which codes for the last enzyme in the violacein biosynthetic pathway - an FAD dependent monooxygenase, which hydroxylates the product | + | This part is composited from the Gli1 zinc finger gene([[Part:BBa_K165007]]) and the ''vio''C gene, which codes for the last enzyme in the violacein biosynthetic pathway - an FAD dependent monooxygenase, which hydroxylates the product of the VioD enzyme to violacein. The resulting proteins are connected with a glycine serine linker. Therefore, the product of the DNA sequence is a chimeric VioC enzyme, which binds to a specific DNA sequence. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 01:30, 24 October 2010
Gli1_vioC
This part is composited from the Gli1 zinc finger gene(Part:BBa_K165007) and the vioC gene, which codes for the last enzyme in the violacein biosynthetic pathway - an FAD dependent monooxygenase, which hydroxylates the product of the VioD enzyme to violacein. The resulting proteins are connected with a glycine serine linker. Therefore, the product of the DNA sequence is a chimeric VioC enzyme, which binds to a specific DNA sequence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1869
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]