Difference between revisions of "Part:BBa K323134:Design"

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===Design Notes===
 
===Design Notes===
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The Zif268 zinc finger sequence was ligated into an existing BioBrick vector containing the Elowitz RBS site (Part:BBa_B0034). The ''vio''B gene was obtained by PCR from an existing BioBrick of violacein operon (Part BBa_K274002) and ligated as a back insert into the RBS_zinc-finger construct.
+
The Zif268 zinc finger sequence was ligated into an existing BioBrick vector containing the Elowitz RBS site ([[Part:BBa_B0034]]). The ''vio''B gene was obtained by PCR from an existing BioBrick of violacein operon ([[Part:BBa_K274002]]) and ligated as a back insert into the RBS_zinc-finger construct.
  
 
===Source===
 
===Source===

Revision as of 21:18, 23 October 2010

Zif268_vioB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3309
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2834
    Illegal AgeI site found at 3030
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Zif268 zinc finger sequence was ligated into an existing BioBrick vector containing the Elowitz RBS site (Part:BBa_B0034). The vioB gene was obtained by PCR from an existing BioBrick of violacein operon (Part:BBa_K274002) and ligated as a back insert into the RBS_zinc-finger construct.

Source

The vioB sequence was obtained by PCR from an existing part, which was designed by the Cambridge 2009 iGEM team (Part:BBa_K274002). The Zif268 zinc finger is a synthetic gene, which our team designed and ordered from GeneArt.

References