Difference between revisions of "Part:BBa K323135:Design"

 
(→‎Design Notes)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K323135 short</partinfo>
 
<partinfo>BBa_K323135 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
a
+
Part BBa_K323134 was ligated into a vector containing part BBa_K323085 as a back insert. The resulting fragment was then ligated into the ''Bsa''I cloning site of part BBa_K323110.
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Revision as of 18:16, 23 October 2010

VioA and VioB enzymes fused with zinc fingers under pBAD promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1204
    Illegal NheI site found at 2568
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 6132
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5657
    Illegal AgeI site found at 979
    Illegal AgeI site found at 5853
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

Part BBa_K323134 was ligated into a vector containing part BBa_K323085 as a back insert. The resulting fragment was then ligated into the BsaI cloning site of part BBa_K323110.

Source

a

References