Difference between revisions of "Part:BBa K323087"
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<partinfo>BBa_K323087 short</partinfo>
 
<partinfo>BBa_K323087 short</partinfo>
  
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This part is composited from the HivC zinc finger gene(Part:BBa_K165006) and the vioD gene, which codes for the fourth enzyme in the violacein biosynthetic pathway. The resulting proteins are connected with a glycine serine linker. Therefore, the product of the DNA sequence is a chimeric VioD enzyme, which binds to a specific DNA sequence.
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This part is composited from the HivC zinc finger gene(Part:BBa_K165006) and the vioD gene, which codes for the fourth enzyme in the violacein biosynthetic pathway - an FAD dependent monooxygenase, that acts by hydroxylation of intermediates and passes them on to the VioC enzyme, which forms violacein. The resulting proteins are connected with a glycine serine linker. Therefore, the product of the DNA sequence is a chimeric VioD enzyme, which binds to a specific DNA sequence.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:06, 23 October 2010

HivC_vioD

This part is composited from the HivC zinc finger gene(Part:BBa_K165006) and the vioD gene, which codes for the fourth enzyme in the violacein biosynthetic pathway - an FAD dependent monooxygenase, that acts by hydroxylation of intermediates and passes them on to the VioC enzyme, which forms violacein. The resulting proteins are connected with a glycine serine linker. Therefore, the product of the DNA sequence is a chimeric VioD enzyme, which binds to a specific DNA sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 90
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1233
    Illegal SapI.rc site found at 1308