Difference between revisions of "Part:BBa K323131:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | a | + | The PBSII zinc finger sequence was ligated into an existing BioBrick vector containing the Elowitz RBS site (Part:BBa_B0034). The vioE gene was obtained by PCR from an existing BioBrick of violacein operon (Part BBa_K274002) and ligated as a back insert into the RBS_zinc-finger construct. |
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===Source=== | ===Source=== | ||
Revision as of 16:42, 23 October 2010
PBSII_vioE
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The PBSII zinc finger sequence was ligated into an existing BioBrick vector containing the Elowitz RBS site (Part:BBa_B0034). The vioE gene was obtained by PCR from an existing BioBrick of violacein operon (Part BBa_K274002) and ligated as a back insert into the RBS_zinc-finger construct.
Source
a