Difference between revisions of "Part:BBa K323087:Design"

(→‎Source)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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The HivC zinc finger sequence (Part: BBa_K165006) (with a previously added linker sequence) was ligated into an existing BioBrick vector containing the Elowitz RBS site (Part:BBa_B0034). The vioC gene was obtained by PCR from an existing BioBrick of violacein operon (Part BBa_K274002) and ligated as a back insert into the RBS_zinc-finger construct.
+
The HivC zinc finger sequence (Part: BBa_K165006) (with a previously added linker sequence) was ligated into an existing BioBrick vector containing the Elowitz RBS site (Part:BBa_B0034). The vioD gene was obtained by PCR from an existing BioBrick of violacein operon (Part BBa_K274002) and ligated as a back insert into the RBS_zinc-finger construct.
  
 
===Source===
 
===Source===

Revision as of 16:32, 23 October 2010

HivC_vioD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 90
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1233
    Illegal SapI.rc site found at 1308


Design Notes

The HivC zinc finger sequence (Part: BBa_K165006) (with a previously added linker sequence) was ligated into an existing BioBrick vector containing the Elowitz RBS site (Part:BBa_B0034). The vioD gene was obtained by PCR from an existing BioBrick of violacein operon (Part BBa_K274002) and ligated as a back insert into the RBS_zinc-finger construct.

Source

The vioD sequence was obtained by PCR from an existing part, which was designed by the Cambridge 2009 iGEM team (Part:BBa_K274002). The HivC zinc finger was also obtained from the Registry of parts (Part:BBa_K165006).

References