Difference between revisions of "Part:BBa K323086:Design"

(→‎Source)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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The Gli1 zinc finger sequence (with a previously added linker sequence) was ligated into an existing BioBrick vector containing the Elowitz RBS site (Part:BBa_B0034). The vioC gene was obtained by PCR from an existing BioBrick of violacein operon (Part BBa_K274002) and ligated as a back insert into the RBS_zinc-finger construct.
+
The Gli1 zinc finger sequence (Part: BBa_K165007) (with a previously added linker sequence) was ligated into an existing BioBrick vector containing the Elowitz RBS site (Part:BBa_B0034). The vioC gene was obtained by PCR from an existing BioBrick of violacein operon (Part BBa_K274002) and ligated as a back insert into the RBS_zinc-finger construct.
  
 
===Source===
 
===Source===

Revision as of 16:31, 23 October 2010

Gli1_vioC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1869
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Gli1 zinc finger sequence (Part: BBa_K165007) (with a previously added linker sequence) was ligated into an existing BioBrick vector containing the Elowitz RBS site (Part:BBa_B0034). The vioC gene was obtained by PCR from an existing BioBrick of violacein operon (Part BBa_K274002) and ligated as a back insert into the RBS_zinc-finger construct.

Source

The vioB sequence was obtained by PCR from an existing part, which was designed by the Cambridge 2009 iGEM team (Part:BBa_K274002). The Gli1 zinc finger was also obtained from the Registry of parts (Part:BBa_K165007).

References