Difference between revisions of "Part:BBa K330002:Design"
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== '''Design notes''' ==
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'''Design notes'''
  
  
  
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The gusA coding sequence was amplified by PCR with plasmid PBI121 (Kmr) as the template. Restriction enzyme cutting sites EcoRI, XbaI, SpeI and PstI was added in the overhangs of two primers following the standard assembly requirements.  
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The gusA coding sequence was amplified by PCR with plasmid PBI121 (Kmr) as the template. Restriction enzyme cutting sites EcoRI, XbaI, SpeI and PstI were added in the overhangs of two primers following the standard assembly requirements.  
  
  

Latest revision as of 16:10, 23 October 2010

gusA reporter gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design notes


The gusA coding sequence was amplified by PCR with plasmid PBI121 (Kmr) as the template. Restriction enzyme cutting sites EcoRI, XbaI, SpeI and PstI were added in the overhangs of two primers following the standard assembly requirements.


Below are primer sequences for the PCR:

gusA F GGTGCCCGGGATGTTACGTCCTGTAGAAACCC

gusA R ATACCTGCAGACTAGTTTATTGTTTGCCTCCCTGC