Difference between revisions of "Part:BBa K330000:Experience"
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Revision as of 15:52, 23 October 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K330000
To demonstrate that this biobirck, “FLAG-tag encoding sequence flanked by standard BioBrick™ prefix and suffix”, works as expected, a construct FLAGtag – EGFP was built and ligated into mammalian expression vector pUHD-P3. HtTA1 cells were transfected with this plasmid and observed under florescence microscope 48 hours after transfection. The cells were then lysed and protein extracts obtained. The extracted proteins were then subjected to SDS-PAGE and blotted with M2 (Sigma, anti-flagtag). HtTA1 cells transfected with empty pUHD-P3 were used as control.
The results are as follows:
User Reviews
UNIQbb0a2eb98cca0817-partinfo-00000001-QINU UNIQbb0a2eb98cca0817-partinfo-00000002-QINU