Difference between revisions of "Part:BBa K323085:Design"

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===Design Notes===
 
===Design Notes===
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This part is composited from the Blues zinc finger gene(Part:BBa_K323136) and the ''vio''A gene, which codes for the first enzyme in the violacein biosynthetic pathway. The resulting proteins are connected with a glycine serine linker. Therefore, the product of the DNA sequence is a chimeric VioA enzyme, which binds to a specific DNA sequence.
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The Blues zinc finger sequence was ligated into an existing BioBrick vector containing the Elowitz RBS site (Part:BBa_B0034). The ''vio''A gene was obtained by PCR from an existing BioBrick of violacein operon (Part BBa_K274002) and ligated as a back insert into the RBS_zinc-finger construct.
  
 
===Source===
 
===Source===

Revision as of 15:19, 23 October 2010

Blues_vioA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1359
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Blues zinc finger sequence was ligated into an existing BioBrick vector containing the Elowitz RBS site (Part:BBa_B0034). The vioA gene was obtained by PCR from an existing BioBrick of violacein operon (Part BBa_K274002) and ligated as a back insert into the RBS_zinc-finger construct.

Source

The vioA sequence was obtained by PCR from an existing part, which was designed by the Cambridge 2009 iGEM team (Part:BBa_K274002). The Blues zinc finger is a synthetic gene, which our team designed and ordored from GeneArt.

References