Difference between revisions of "Part:BBa K358010:Design"
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===Design Notes=== | ===Design Notes=== | ||
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We performed three steps of PCR to prepare this part. | We performed three steps of PCR to prepare this part. | ||
Latest revision as of 11:44, 23 October 2010
anti-killer gene with GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 962
Design Notes
We performed three steps of PCR to prepare this part.
First, we got S gene from lambda phage DNA: λ -Hind Ⅲ digest, and inserted into the plasmid, BBa_E0840.
Then, performed the point mutation PCR, the same to the point mutation PCR on SRRz.
Finally, we deleted a transmembrane domain, TMD1, and constructed the anti-killergene: SΔTMD1.
Source
λ -Hind Ⅲ digest (product of TaKaRa Bio) and BBa_E0240
References
R. White, T. A. T. Tran, C. A. Dankenbring, J. Deaton, and R. Young, “The N-terminal transmembrane domain of lambda S is required for holin but not antiholin function,” Journal of Bacteriology, vol. 192, no. 3, pp. 725-733, Feb. 2010.