Difference between revisions of "Part:BBa K358012:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
Made point mutation and deletion PCR on S gene and prepared SΔTMD1.
+
We prepared this part with deletion PCR.  Deleted GFP and terminator from BBa_K358010, but we missed the design of primers so that the scar is still remain in this part.
  
 
===Source===
 
===Source===

Revision as of 09:58, 23 October 2010

SΔTMD1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We prepared this part with deletion PCR. Deleted GFP and terminator from BBa_K358010, but we missed the design of primers so that the scar is still remain in this part.

Source

λ -Hind Ⅲ digest(product of TaKaRa Bio)

References