Difference between revisions of "Part:BBa K314100"
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<partinfo>BBa_K314100 short</partinfo>
 
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High constitutive protein expression insert includes f1 origin k314110, a high expression promoter J23100, and the  Elowitz standard RBS B0034.  When ligated with a protein coding sequence at the SpeI site the scar site created will create proper spacing for protein expression.  
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High constitutive protein expression insert includes f1 origin [https://parts.igem.org/Part:BBa_K314110 K314110], a high expression promoter [https://parts.igem.org/Part:BBa_J23100 J23100], and the  Elowitz standard RBS [https://parts.igem.org/Part:BBa_B0034 B0034].  When ligated with a protein coding sequence at the SpeI site the scar site created will create proper spacing for protein expression.  
  
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K314100 parameters</partinfo>
 
<partinfo>BBa_K314100 parameters</partinfo>
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GFP expression assay shows activity of K314100 as compaired to other protein expression cassettes.
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[[Image:Washington2010_vector_assay_data.png|thumb|left|650px|'''Figure 1.  GFP fluorescence was measured (ex. 485, em. 525) and normalized to cell density, read as the optical density at 600nm.''']]

Revision as of 04:12, 23 October 2010

High constitutive expression cassette

High constitutive protein expression insert includes f1 origin K314110, a high expression promoter J23100, and the Elowitz standard RBS B0034. When ligated with a protein coding sequence at the SpeI site the scar site created will create proper spacing for protein expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 470
    Illegal NheI site found at 493
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

GFP expression assay shows activity of K314100 as compaired to other protein expression cassettes.

Figure 1. GFP fluorescence was measured (ex. 485, em. 525) and normalized to cell density, read as the optical density at 600nm.