Difference between revisions of "Part:BBa K314200"

Revision as of 03:12, 23 October 2010

Toxin Tse2

A toxic protein originating from Pseudomonas aeruginosa that has been shown to arrest growth in both prokaryotic and eukaryotic cells when expressed intracelluarly. It is a substrate of the Pseudomonas aeruginosa type 6 secretion system. Tse2 has an immunity protein, Tsi2, that, when expressed in conjunction with Tse2 prevents cell death. [http://2010.igem.org/Team:Washington/Gram_Negative Find more information here on our wiki!.]

Reference showing that Tse2 is toxic, Tsi2 is the antitoxin, and that these proteins are transferred into target Gram-negative organisms via a secretion system: "A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria" [http://www.ncbi.nlm.nih.gov/pubmed/20114026]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 196
Illegal NgoMIV site found at 253
Illegal NgoMIV site found at 378
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K314200 short</partinfo>
-A toxic protein originating from ''Pseudomonas aeruginosa'' that has been shown to arrest growth in both prokaryotic and eukaryotic cells when expressed intracelluarly. It is a substrate of the ''Pseudomonas aeruginosa'' type 6 secretion system. Tse2 has an immunity protein, [https://parts.igem.org/wiki/index.php?title=Part:BBa_K314201 Tsi2], that, when expressed in conjunction with Tse2 prevents cell death.
+A toxic protein originating from ''Pseudomonas aeruginosa'' that has been shown to arrest growth in both prokaryotic and eukaryotic cells when expressed intracelluarly. It is a substrate of the ''Pseudomonas aeruginosa'' type 6 secretion system. Tse2 has an immunity protein, [https://parts.igem.org/wiki/index.php?title=Part:BBa_K314201 Tsi2], that, when expressed in conjunction with Tse2 prevents cell death.  [http://2010.igem.org/Team:Washington/Gram_Negative Find more information here on our wiki!.]
 Reference showing that Tse2 is toxic, Tsi2 is the antitoxin, and that these proteins are transferred into target Gram-negative organisms via a secretion system: "A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria" [http://www.ncbi.nlm.nih.gov/pubmed/20114026]