Difference between revisions of "Part:BBa K404108:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K404108 short</partinfo>
 
<partinfo>BBa_K404108 short</partinfo>
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===Design Notes===
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<h3>Design Notes</h3>
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For designing the BioBrick part, the iGEM team Freiburg used primers in the RFC10 standard and performed a PCR. The PCR product was digested using XbaI and PstI followed by ligation into the iGEM standard plasmid pSB1C3.
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===Source===
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<h3>Source</h3>
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The plasmid was PCR amplified using the vector pAAV_MCS as DNA template.
  
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<h3>References</h3>
  
 
===References===
 
===References===
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<b>Danckwardt, S., Hentze, M. W., & Kulozik, A. E.</b> (2008). 3' end mRNA processing: molecular mechanisms and implications for health and disease. The EMBO journal, 27(3), 482-98. doi: 10.1038/sj.emboj.7601932.<br />
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<b>Millevoi, S., Loulergue, C., Dettwiler, S., Karaa, S. Z., Keller, W., Antoniou, M., et al.</b> (2006). An interaction between U2AF 65 and CF I(m) links the splicing and 3' end processing machineries. The EMBO journal, 25(20), 4854-64. doi: 10.1038/sj.emboj.7601331.

Revision as of 02:44, 23 October 2010

hGH terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 387


Design Notes

For designing the BioBrick part, the iGEM team Freiburg used primers in the RFC10 standard and performed a PCR. The PCR product was digested using XbaI and PstI followed by ligation into the iGEM standard plasmid pSB1C3.

Source

The plasmid was PCR amplified using the vector pAAV_MCS as DNA template.

References

References

Danckwardt, S., Hentze, M. W., & Kulozik, A. E. (2008). 3' end mRNA processing: molecular mechanisms and implications for health and disease. The EMBO journal, 27(3), 482-98. doi: 10.1038/sj.emboj.7601932.
Millevoi, S., Loulergue, C., Dettwiler, S., Karaa, S. Z., Keller, W., Antoniou, M., et al. (2006). An interaction between U2AF 65 and CF I(m) links the splicing and 3' end processing machineries. The EMBO journal, 25(20), 4854-64. doi: 10.1038/sj.emboj.7601331.