Difference between revisions of "Part:BBa K360022"

Revision as of 23:52, 21 October 2010

RBS + cI inverter + GFP

The expression of the trp operon is repressed 300-fold by TrpR. TrpR binds at the trpLp promoter, possibly directly blocking binding by RNA polymerase Klig LS,1988.

The blue light sensor LovTAP is a chiameric protein that contains the TrpR DNA-binding domain, so that is expected to regulate trpL promoter as the native TrpR transcriptional regulator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1658

References

O'Kane, D.J., Woodward, B., Lee, J., and Prasher, D.C. 1990. Borrowed proteins in bacterial bioluminescence. Proc. Natl. Acad. Sci. USA 88, 1100-1104.

Lee, J., Wang, Y., and Gibson, B.G. 1991. Electronic excitation transfer in the complex of lumazine protein with bacterial bioluminescence intermediates. Biochemistry 30, 6825-6835.

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 ===References===
-Klig LS., Carey J., Yanofsky C., 1988, trp repressor interactions with the trp aroH and trpR operators. Comparison of repressor binding in vitro and repression in vivo., J Mol Biol. 202(4):769-77.
-[http://regulondb.ccg.unam.mx/operon?term=ECK120014577&format=jsp#trpLp RegulonDB]
+* O'Kane, D.J., Woodward, B., Lee, J., and Prasher, D.C. 1990. Borrowed proteins in bacterial bioluminescence. ''Proc. Natl. Acad. Sci. USA'' '''88''', 1100-1104.
+* Lee, J., Wang, Y., and Gibson, B.G. 1991. Electronic excitation transfer in the complex of lumazine protein with bacterial bioluminescence intermediates. ''Biochemistry'' '''30''', 6825-6835.